Ibnosina Journal of Medicine and Biomedical Sciences

ORIGINAL ARTICLE
Year
: 2019  |  Volume : 11  |  Issue : 3  |  Page : 111--115

Prevalence of multi-drug-resistant tuberculosis among human immunodeficiency virus and nonhuman immunodeficiency virus-positive pulmonary tuberculosis patients of two referral hospitals in Southeast Nigeria


Chinenye Esther Okoro1, Stellamaris Ojiuzor Ibhawaegbele2, Charles Ikechukwu Ezema3, Uchechukwu Anthonia Ezeugwu3, Chukwuma Paulinus Igweagu4, Ogechukwu Calista Dozie-Nwakile5 
1 Department of Medical Microbiology, Medical Laboratory Services, Enugu State University Teaching Hospital, Parklane, Enugu, Nigeria
2 Department of Medical Laboratory Science, Ambrose Alli University, Ekpoma, Edo State, Nigeria
3 Departments of Medical Rehabilitation, Faculty of Health Sciences and Technology, College of Medicine, University of Nigeria, Enugu Campus, Nigeria
4 Department of Community Medicine, Enugu State University Teaching Hospital, Parklane, Enugu, Nigeria
5 Departments of Medical Laboratory Science, Faculty of Health Sciences and Technology, College of Medicine, University of Nigeria, Enugu Campus, Nigeria

Correspondence Address:
Chinenye Esther Okoro
Department of Medical Microbiology, Medical Laboratory Services, Enugu State University Teaching Hospital, Parklane, PMB 1030, Enugu
Nigeria

Introduction: Multidrug-resistant tuberculosis (MDR-TB) is a type of TB that is resistant to the two most effective first-line drugs: rifampicin and isoniazid and it remains a major public health threat, particularly in developing countries. Objectives: To assess the MDR-TB prevalence among human immunodeficiency virus (HIV) and nonHIV positive pulmonary TB patients of two referral hospitals in southeast Nigeria. Materials and Methods: Sputum specimens of individuals presenting with a cough of >2 weeks duration were screened by Ziehl–Neelsen technique for the presence of acid-fast bacilli (AFB). Results: A total of 103 subjects with AFB-positive sputum samples were recruited from the two referral hospitals and HIV-1/2 antibodies were screened using serial algorithm method. The positive sputum samples were subjected to Xpert MTB/RIF assay (GeneXpert®, Cepheid, USA) and cultured on the Lowenstein–Jensen medium for 42 days at 37°C. Drug susceptibility testing was done on the isolates using the nitrate reduction assay. Eighty-three (80.6%) of the isolates were obtained from culture after suspected colonies were subjected to morphological, biochemical, and immunological tests and of the 83 (80.6%) samples analyzed using Xpert MTB/RIF assay 45 (67.2%) were rifampicin-resistant. The prevalence of culture-positive TB was higher in the HIV-negative sub-population (82.02%) when compared with the HIV-positive participants (71.40%). The rate of MDR-TB was high among HIV-positive patients though not statistically significant. HIV positive patients showed prevalence of (66.70%), whereas HIV-negative patients had (42.60%). Conclusions: The World Health Organization estimated that 26% of patients with TB infection in Nigeria are HIV-positive and the alarming evidence of MDR-TB prevalence in this study calls for close monitoring of the prevalence of drug resistance, especially in HIV-infected population.


How to cite this article:
Okoro CE, Ibhawaegbele SO, Ezema CI, Ezeugwu UA, Igweagu CP, Dozie-Nwakile OC. Prevalence of multi-drug-resistant tuberculosis among human immunodeficiency virus and nonhuman immunodeficiency virus-positive pulmonary tuberculosis patients of two referral hospitals in Southeast Nigeria.Ibnosina J Med Biomed Sci 2019;11:111-115


How to cite this URL:
Okoro CE, Ibhawaegbele SO, Ezema CI, Ezeugwu UA, Igweagu CP, Dozie-Nwakile OC. Prevalence of multi-drug-resistant tuberculosis among human immunodeficiency virus and nonhuman immunodeficiency virus-positive pulmonary tuberculosis patients of two referral hospitals in Southeast Nigeria. Ibnosina J Med Biomed Sci [serial online] 2019 [cited 2019 Dec 13 ];11:111-115
Available from: http://www.ijmbs.org/article.asp?issn=1947-489X;year=2019;volume=11;issue=3;spage=111;epage=115;aulast=Okoro;type=0